PDBredo - a site where they re-refined everyone's structures ProtBud - PC program to evaluate multimer contacts and compare to related structures. PISA-Protein Interfaces, Surfaces and Assemblies (using PDB or new coordinates) SCOP - Structural Classification of Proteins AcaClone pDRAW32 Freeware DNA cloning, analysis and visualisation. #SERIAL CLONER CLONE IN OLIGO SERIAL#MUSTANG - A MUtiple STructure AligNment alGorithm Serial Cloner reads and write DNA Strider-compatible files and import and export files. Indonesia - USF's biological sequence analysis (not installed) - multiple sequence alignments STRAP - Multiple Structural/Sequence Alignment POSA - Partial Order Structural Alignments MATRAS - MArkovian TRAnsition of Structure evolution - does multiple alignments STAMP - Structural Alignment of Multiple Proteins (several choices) SSM - Secondary Structure Matching - multiple PDB comparison and sequence alignments. MSDServer - Macromolecular Structure Database (Can provide %similarity cutoff to see entire structural family) VAST - (NCBI) Vector Alignment of Search Tool - protein structural neighbors IP6-activated fusion proteins: maps, paper, protocol, Vector sequence (2000) A modular Set of Prokaryotic and Eukaryotic Expression Vectors Analytical Biochemistry 277, 109-120): pKA8 complete DNA sequence, pKA8 Cloning Map, Original Cloning Paper description Yeast Vectors: pYES2.0, pTF162, Stuff from Tim's lab about this series of vectorsīaculoVirus Vectors: pAcGP67-A for expression with a signal sequence for secretion PGEX Vectors: pGEXvectors, pGST-parallel, pGST-PP and pGST-TEV vectors from Steve (additional GST-PP and TEV vector sequences) Modified pET Vectors: pET11a-His(7)-PP, pET16b-TEV #SERIAL CLONER CLONE IN OLIGO FULL#PET151-TOPO Cloning site map and full vector mapĭUET Vectors: pACYDuet-1, pCDFDuet-1, pCOLADuet-1, pRSFDuet-1, pETDuet-1 Novagen's pET vector manual (expression, toxcicity tips, etc) Diluting the ds Oligo To clone your ds oligo into pENTR/U6 or pENTR/H1/TO, you must dilute the 50 M stock to a final concentration of 5 nM (i.e. SLIC cloning, pGST-PP-GFP and pHIS-SUMO-GFP Store the remainder of the 50 M ds oligo mixture at -20C. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, pMCSG21 Yeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7 PDEST14 pDEST15, pDEST17, pENTR/TEV/D-TOPO, MCS PENTRL1pLaclacZaL4, pENTRL1pLaclacZaR5, pENTR元pLactetL2, pENTRL5LacIL4, pENTRL5pLacSpecL2, pENTRR4pLacSpecR3
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |